Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations.

ABSTRACT: Advanced light microscopy is an important tool for nanostructure analysis of chromatin. In this report we present a general concept for Single Molecule localization Microscopy (SMLM) super-resolved imaging of DNA-binding dyes based on modifying the properties of DNA and the dye. By careful adjustment of the chemical environment leading to local, reversible DNA melting and hybridization control over the fluorescence signal of the DNA-binding dye molecules can be introduced. We postulate a transient binding as the basis for our variation of binding-activated localization microscopy (BALM). We demonstrate that several intercalating and minor-groove binding DNA dyes can be used to register (optically isolate) only a few DNA-binding dye signals at a time. To highlight this DNA structure fluctua

SUBMITTER: Szczurek A 

PROVIDER: S-EPMC5416826 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature