Triiodothyronine promotes cardiac differentiation and maturation of embryonic stem cells via the classical genomic pathway.

ABSTRACT: Embryonic stem cells (ESCs) can differentiate into functional cardiomyocytes and thus represent a promising cell source for cardiac regenerative therapy. Nevertheless, the therapeutic application of ESC-derived cardiomyocytes is limited by the low efficacy of the current protocol for cardiac differentiation and their immature phenotypes. Although thyroid hormone is essential for normal cardiac development and function, its role in the cardiac differentiation of ESCs, as well as the maturation of ESC-derived cardiomyocytes, remains unclear. In this study, we examined the cardiac differentiation of murine ESCs in the presence of T(3) for 7 d using flow cytometry, RT-PCR, cellular electrophysiology study, and confocal calcium imaging. Compared with control conditions, T(3) supplementation inc

SUBMITTER: Lee YK 

PROVIDER: S-EPMC5417405 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature