Sendai Virus Mucosal Vaccination Establishes Lung-Resident Memory CD8 T Cell Immunity and Boosts BCG-Primed Protection against TB in Mice.

ABSTRACT: Accumulating evidence has shown the protective role of CD8⁺ T cells in vaccine-induced immunity against Mycobacterium tuberculosis (Mtb) despite controversy over their role in natural immunity. However, the current vaccine BCG is unable to induce sufficient CD8⁺ T cell responses, especially in the lung. Sendai virus, a respiratory RNA virus, is here engineered firstly as a novel recombinant anti-TB vaccine (SeV85AB) that encodes Mtb immuno-dominant antigens, Ag85A and Ag85B. A single mucosal vaccination elicited potent antigen-specific T cell responses and a degree of protection against Mtb challenge similar to the effect of BCG in mice. Depletion of CD8⁺ T cells abrogated the protective immunity afforded by SeV85AB vaccination. Interestingly, only SeV85AB

SUBMITTER: Hu Z 

PROVIDER: S-EPMC5417795 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature