Protein Footprinting Comes of Age: Mass Spectrometry for Biophysical Structure Assessment.

ABSTRACT: Protein footprinting mediated by mass spectrometry has evolved over the last 30 years from proof of concept to commonplace biophysics tool, with unique capabilities for assessing structure and dynamics of purified proteins in physiological states in solution. This review outlines the history and current capabilities of two major methods of protein footprinting: reversible hydrogen-deuterium exchange (HDX) and hydroxyl radical footprinting (HRF), an irreversible covalent labeling approach. Technological advances in both approaches now permit high-resolution assessments of protein structure including secondary and tertiary structure stability mediated by backbone interactions (measured via HDX) and solvent accessibility of side chains (measured via HRF). Applications across many academic fie

SUBMITTER: Wang L 

PROVIDER: S-EPMC5417815 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature