Covariation of Peptide Abundances Accurately Reflects Protein Concentration Differences.

ABSTRACT: Most implementations of mass spectrometry-based proteomics involve enzymatic digestion of proteins, expanding the analysis to multiple proteolytic peptides for each protein. Currently, there is no consensus of how to summarize peptides' abundances to protein concentrations, and such efforts are complicated by the fact that error control normally is applied to the identification process, and do not directly control errors linking peptide abundance measures to protein concentration. Peptides resulting from suboptimal digestion or being partially modified are not representative of the protein concentration. Without a mechanism to remove such unrepresentative peptides, their abundance adversely impacts the estimation of their protein's concentration. Here, we present a relative quantification

SUBMITTER: Zhang B 

PROVIDER: S-EPMC5417831 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature