Airway eosinophil migration into lymph nodes in mice depends on leukotriene C₄.

ABSTRACT:

Background

We previously demonstrated in mice that airway eosinophils traffic from the airway lumen into lung-draining paratracheal lymph nodes. However, mechanisms whereby eosinophils traverse from the lungs and home to paratracheal lymph nodes remain unclear. We investigated roles of cysteinyl leukotrienes in mediating eosinophil trafficking from lungs to paratracheal lymph nodes.

Methods

The expression of CCR7 was determined by flow cytometry. Transwell assays were used to test chemotactic responses of leukotriene C₄ synthase-deficient and control airway eosinophils to the chemokine CCL19 ex vivo. Eosinophils from the spleens of IL-5 transgenic mice, fluorescently labeled ex vivo, were intratracheally injected into ovalbumin-sensitized and ovalbumin aerosol-cha

SUBMITTER: Wang HB 

PROVIDER: S-EPMC5418101 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature