Expression of the inhibitory receptor NKG2A correlates with increased liver and splenic NK cell response to activating receptor engagement.

ABSTRACT:

Introduction

Natural killer (NK) cells play a critical role in the innate immune response to viruses and tumors, and comprise a large proportion of the hepatic lymphocyte population. They must remain tolerant to non-pathogenic antigens while protecting the host from harmful agents. Herein, we investigate how the NK cell response to activation receptor engagement is altered in the liver.

Methods

In this study, we assess IFN-γ production and degranulation of splenic NK cells and selected subsets of liver NK cells. Flow cytometry (FCM) was used to asses IFN-γ production and degranulation following stimulation of the NK cells with plate bound antibodies to activating receptors.

Results

We show that smaller percentages of hepatic NK cells produce interferon (IFN)-γ and/or

SUBMITTER: Meyer CE 

PROVIDER: S-EPMC5418142 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature