Predicting genome terminus sequences of Bacillus cereus-group bacteriophage using next generation sequencing data.

ABSTRACT:

Background

Most tailed bacteriophages (phages) feature linear dsDNA genomes. Characterizing novel phages requires an understanding of complete genome sequences, including the definition of genome physical ends.

Result

We sequenced 48 Bacillus cereus phage isolates and analyzed Next-generation sequencing (NGS) data to resolve the genome configuration of these novel phages. Most assembled contigs featured reads that mapped to both contig ends and formed circularized contigs. Independent assemblies of 31 nearly identical I48-like Bacillus phage isolates allowed us to observe that the assembly programs tended to produce random cleavage on circularized contigs. However, currently available assemblers were not capable of reporting the underlying phage genome configuration from seq

SUBMITTER: Chung CH 

PROVIDER: S-EPMC5418689 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature