Tracking microbial colonization in fecal microbiota transplantation experiments via genome-resolved metagenomics.

ABSTRACT:

Background

Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridium difficile infection and shows promise for treating other medical conditions associated with intestinal dysbioses. However, we lack a sufficient understanding of which microbial populations successfully colonize the recipient gut, and the widely used approaches to study the microbial ecology of FMT experiments fail to provide enough resolution to identify populations that are likely responsible for FMT-derived benefits.

Methods

We used shotgun metagenomics together with assembly and binning strategies to reconstruct metagenome-assembled genomes (MAGs) from fecal samples of a single FMT donor. We then used metagenomic mapping to track the occurrence and distribution patterns o

SUBMITTER: Lee STM 

PROVIDER: S-EPMC5418705 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature