Circulating mutational portrait of cancer: manifestation of aggressive clonal events in both early and late stages.

ABSTRACT:

Background

Solid tumors residing in tissues and organs leave footprints in circulation through circulating tumor cells (CTCs) and circulating tumor DNAs (ctDNA). Characterization of the ctDNA portraits and comparison with tumor DNA mutational portraits may reveal clinically actionable information on solid tumors that is traditionally achieved through more invasive approaches.

Methods

We isolated ctDNAs from plasma of patients of 103 lung cancer and 74 other solid tumors of different tissue origins. Deep sequencing using the Guardant360 test was performed to identify mutations in 73 clinically actionable genes, and the results were associated with clinical characteristics of the patient. The mutation profiles of 37 lung cancer cases with paired ctDNA and tumor genomic DNA seq

SUBMITTER: Yang M 

PROVIDER: S-EPMC5418716 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature