Rhinovirus C targets ciliated airway epithelial cells.

ABSTRACT:

Background

The Rhinovirus C (RV-C), first identified in 2006, produce high symptom burdens in children and asthmatics, however, their primary target host cell in the airways remains unknown. Our primary hypotheses were that RV-C target ciliated airway epithelial cells (AECs), and that cell specificity is determined by restricted and high expression of the only known RV-C cell-entry factor, cadherin related family member 3 (CDHR3).

Methods

RV-C15 (C15) infection in differentiated human bronchial epithelial cell (HBEC) cultures was assessed using immunofluorescent and time-lapse epifluorescent imaging. Morphology of C15-infected differentiated AECs was assessed by immunohistochemistry.

Results

C15 produced a scattered pattern of infection, and infected cells were shed f

SUBMITTER: Griggs TF 

PROVIDER: S-EPMC5418766 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature