Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells.

ABSTRACT:

Background

Excessive accumulation of adipose tissue in cultured fish is an outstanding problem in aquaculture. To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. Therefore, the transcriptomic profile at different time points (days 3, 8, 15 and 21) along primary culture development of rainbow trout preadipocytes has been investigated using an Agilent trout oligo microarray.

Results

Our analysis identified 4026 genes differentially expressed (fold-change >3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation. Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of cul

SUBMITTER: Bou M 

PROVIDER: S-EPMC5418865 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature