Gamma-glutamyltransferase activity in exosomes as a potential marker for prostate cancer.

ABSTRACT:

Background

Exosomes or extracellular vesicles have the potential as a diagnostic marker for various diseases including cancer. In order to identify novel exosomal markers for prostate cancer (PC), we performed proteomic analysis of exosomes isolated from PC cell lines and examined the usefulness of the marker in patients.

Methods

Exosomes isolated by differential centrifugation from the culture medium of androgen-dependent LNCaP prostate cancer cell line and its sublines of partially androgen-independent C4, androgen-independent C4-2 and bone metastatic C4-2B were subjected to iTRAQ-based proteomic analysis. Exosomes were also isolated by immunocapture and separated by size exclusion chromatography and density gradient centrifugation. Protein expression was determined by Wes

SUBMITTER: Kawakami K 

PROVIDER: S-EPMC5420129 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature