Protein and glycomic plasma markers for early detection of adenoma and colon cancer.

ABSTRACT:

Objective

To discover and confirm blood-based colon cancer early-detection markers.

Design

We created a high-density antibody microarray to detect differences in protein levels in plasma from individuals diagnosed with colon cancer <3 years after blood was drawn (ie, prediagnostic) and cancer-free, matched controls. Potential markers were tested on plasma samples from people diagnosed with adenoma or cancer, compared with controls. Components of an optimal 5-marker panel were tested via immunoblotting using a third sample set, Luminex assay in a large fourth sample set and immunohistochemistry (IHC) on tissue microarrays.

Results

In the prediagnostic samples, we found 78 significantly (t-test) increased proteins, 32 of which were confirmed in the diagnostic samples. F

SUBMITTER: Rho JH 

PROVIDER: S-EPMC5420499 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature