Developmental changes in electrophysiological characteristics of human-induced pluripotent stem cell-derived cardiomyocytes.

ABSTRACT:

Background

Previous studies proposed that throughout differentiation of human induced Pluripotent Stem Cell-derived cardiomyocytes (iPSC-CMs), only 3 types of action potentials (APs) exist: nodal-, atrial-, and ventricular-like.

Objectives

To investigate whether there are precisely 3 phenotypes or a continuum exists among them, we tested 2 hypotheses: (1) During culture development a cardiac precursor cell is present that-depending on age-can evolve into the 3 phenotypes. (2) The predominant pattern is early prevalence of a nodal phenotype, transient appearance of an atrial phenotype, evolution to a ventricular phenotype, and persistence of transitional phenotypes.

Methods

To test these hypotheses, we (1) performed fluorescence-activated cell sorting analysis of nodal

SUBMITTER: Ben-Ari M 

PROVIDER: S-EPMC5421365 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature