Common rs5918 (PlA1/A2) polymorphism in the ITGB3 gene and risk of coronary artery disease.

ABSTRACT:

Introduction

The T to C transition at nucleotide 1565 of the human glycoprotein IIIa (ITGB3) gene represents a genetic polymorphism (PlA1/A2) that can influence both platelet activation and aggregation and that has been associated with many types of disease. Here, we present a newly designed multiplex tetra-primer amplification refractory mutation system - polymerase chain reaction (T-ARMS-PCR) for genotyping a single nucleotide polymorphism (SNP) (dbSNP ID: rs5918) in the human ITGB3 gene.

Material and methods

We set up T-ARMS-PCR for the rs5918 SNP in a single-step PCR and the results were validated by the PCR-RFLP method in 132 coronary artery disease (CAD) patients and 122 unrelated healthy individuals.

Results

Full accordance was found for genotype

SUBMITTER: Khatami M 

PROVIDER: S-EPMC5421535 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature