Succinct workflows for circulating tumor cells after enrichment: From systematic counting to mutational profiling.

ABSTRACT:

Purpose

This study aims to establish a highly adaptable workflow downstream of microfluidic enrichment for facilitating systematic CTC enumeration and genetic discovery.

Methods

To facilitate CTC enumeration, we established a CK/EPCAM-combined immunostaining strategy and an automated CTC analytical pipeline using an open-source image analyzer. By virtue of this workflow, we conducted a pilot study of 56 cancer patients and 21 healthy individuals using a high-throughput spiral microfluidic chip system. To facilitate genetic discovery of somatic mutations in CTCs, we integrated the CTC enumeration into next-generation sequencing and established a straightforward amplicon library comprising diversifier random sequences to sequence CTC samples.

Results

The CTC staining an

SUBMITTER: Wong VC 

PROVIDER: S-EPMC5421802 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature