MiR-21-5p in urinary extracellular vesicles is a novel biomarker of urothelial carcinoma.

ABSTRACT:

Background

Extracellular vesicles are lipid bilayer vesicles containing protein, messengerRNA and microRNA. Cancer cell-derived extracellular vesicles may be diagnostic and therapeutic targets. We extracted extracellular vesicles from urine of urothelial carcinoma patients and the control group to identify cancer-specific microRNAs in urinary extracellular vesicles as new biomarkers.

Materials and methods

microRNA from urinary extracellular vesicles extracted from 6 urothelial carcinoma patients and 3 healthy volunteers was analyzed. We verified candidate microRNAs in an independent cohort of 60 urinary extracellular vesicles samples. To normalize the microRNA expression level in extracellular vesicles, we examined the following in extracellular vesicles: protein concentrati

SUBMITTER: Matsuzaki K 

PROVIDER: S-EPMC5421878 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature