Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping.

ABSTRACT: Colorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%-60% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor (EGFR) agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the main KRAS mutations on the

SUBMITTER: Guarnaccia M 

PROVIDER: S-EPMC5422192 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature