The effects of quercetin on microRNA and inflammatory gene expression in lipopolysaccharide-stimulated bovine neutrophils.

ABSTRACT:

Aim

To investigate gene expression of microRNA (miRNA) milieus (MIRLET7E, MIR17, MIR24-2, MIR146A, and MIR181C), inflammatory cytokine genes (interleukin 1β [IL1B], IL6, CXCL8, and tumor necrosis factor [TNF]), and the pathogen receptor toll-like receptor (TLR4) in bovine neutrophils under quercetin supplementation.

Materials and methods

Isolated bovine neutrophils were incubated with bacterial lipopolysaccharide under quercetin treatment or left untreated. Real-time polymerase chain reaction was performed to determine the expression of the miRNAs and messenger RNA (mRNA) transcripts in neutrophils.

Results

Quercetin-treated neutrophils exhibited a remarkable suppression in MIR24-2, MIR146

SUBMITTER: Chuammitri P 

PROVIDER: S-EPMC5422244 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature