Targeted siRNA delivery reduces nitric oxide mediated cell death after spinal cord injury.

ABSTRACT:

Background

Traumatic spinal cord injury (SCI) includes the primary insult as well as a sequela of biochemical and cellular cascades that amplifies the initial injury. This degenerative process, known as secondary injury, is often mediated by both reactive oxygen and nitrogen species released from damaged cells. Previous data suggests that dysregulated production of nitric oxide via inducible nitric oxide synthase (iNOS) is detrimental to spinal cord recovery. M1 macrophages have been implicated to overexpress iNOS post-SCI. In this work, we propose to inhibit iNOS expression through small interfering RNA (siRNA) complexed chitosan nanoparticles (NPs) that primarily target M1 macrophages.

Methods

siRNA conjugated chitosan complexes were fabricated with and without an antibody

SUBMITTER: Gao W 

PROVIDER: S-EPMC5422875 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature