A robust PCR for the differentiation of potential virulent strains of Haemophilus parasuis.

ABSTRACT:

Background

Haemophilus parasuis is the etiological agent of Glässer's disease in swine. H. parasuis comprises strains with heterogeneous virulence capacity, from non-virulent to highly virulent. Determination of the pathogenic potential of the strains is important for diagnosis and disease control. The virulence-associated trimeric autotransporters (vtaA) genes have been used to predict H. parasuis virulence by PCR amplification of their translocator domains. Here, we report a new and improved PCR designed to detect a different domain of the vtaA genes, the leader sequence (LS) as a diagnostic tool to predict virulence.

Methods

A collection of 360 H. parasuis strains was tested by PCR with LS specific primers. Results of the PCR were compared with the clinical origin of th

SUBMITTER: Galofre-Mila N 

PROVIDER: S-EPMC5422950 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature