Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells.

ABSTRACT:

Background

Epigenome editing is expected to manipulate transcription and cell fates and to elucidate the gene expression mechanisms in various cell types. For functional epigenome editing, assessing the chromatin context-dependent activity of artificial epigenetic modifier is required.

Results

In this study, we applied clustered regularly interspaced short palindromic repeats (CRISPR)-dCas9-based epigenome editing to mouse primary T cells, focusing on the Forkhead box P3 (Foxp3) gene locus, a master transcription factor of regulatory T cells (Tregs). The Foxp3 gene locus is regulated by combinatorial epigenetic modifications, which determine the Foxp3 expression. Foxp3 expression is unstable in transforming growth factor beta (TGF-β)-induced Tregs (iTregs), whi

SUBMITTER: Okada M 

PROVIDER: S-EPMC5422987 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature