MiR-200c and phospho-AKT as prognostic factors and mediators of osteosarcoma progression and lung metastasis.

ABSTRACT: Lung metastasis is the major cause of death in osteosarcoma patients. However, molecular mechanisms underlying this metastasis remain poorly understood. To identify key molecules related with pulmonary metastasis of pediatric osteosarcomas, we analyzed high-throughput miRNA expression in a cohort of 11 primary tumors and 15 lung metastases. Results were further validated with an independent cohort of 10 primary tumors and 6 metastases. In parallel, we performed immunohistochemical analysis of activated signaling pathways in 36 primary osteosarcomas. Only phospho-AKT associated with lower overall survival in primary tumors, supporting its role in osteosarcoma progression. CTNNB1 expression also associated with lower overall survival but was not strong enough to be considered an independent

SUBMITTER: Berlanga P 

PROVIDER: S-EPMC5423177 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature