Global re-wiring of p53 transcription regulation by the hepatitis B virus X protein.

ABSTRACT:

Background

The tumour suppressor p53 is a central player in transcription regulation and cell fate determination. By interacting with p53 and altering its sequence-specific binding to the response elements, the hepatitis B virus X protein (HBx) was reported to re-direct p53 regulation of some genes.

Results

Coupling massively parallel deep sequencing with p53 chromatin immunoprecipitation, we demonstrate that HBx modulates global p53 site selection and that this was strongly influenced by altered interaction with transcription co-factors/co-regulators as well as post-translational modifications. Specifically, HBx attenuated p53-TBP-RB1 transcription complex recruitment and interaction and this was associated with hyper-phosphorylation of p53 at serine 315 by HBx. Concurrentl

SUBMITTER: Chan C 

PROVIDER: S-EPMC5423203 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature