Genotyping-by-Sequencing and Its Exploitation for Forage and Cool-Season Grain Legume Breeding.

ABSTRACT: Genotyping-by-Sequencing (GBS) may drastically reduce genotyping costs compared with single nucleotide polymorphism (SNP) array platforms. However, it may require optimization for specific crops to maximize the number of available markers. Exploiting GBS-generated markers may require optimization, too (e.g., to cope with missing data). This study aimed (i) to compare elements of GBS protocols on legume species that differ for genome size, ploidy, and breeding system, and (ii) to show successful applications and challenges of GBS data on legume species. Preliminary work on alfalfa and Medicago truncatula suggested the greater interest of ApeKI over PstI:MspI DNA digestion. We compared KAPA and NEB Taq polymerases in combination with primer extensions that

SUBMITTER: Annicchiarico P 

PROVIDER: S-EPMC5423274 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature