Differential sex-specific effects of oxygen toxicity in human umbilical vein endothelial cells.

ABSTRACT: Despite the well-established sex-specific differences in the incidence of bronchopulmonary dysplasia (BPD), the molecular mechanism(s) behind these are not completely understood. Pulmonary angiogenesis is critical for alveolarization and arrest in vascular development adversely affects lung development. Human neonatal umbilical vein endothelial cells (HUVECs) provide a robust in vitro model for the study of endothelial cell physiology and function. Male and Female HUVECs were exposed to room air (21% O₂, 5% CO₂) or hyperoxia (95% O₂, 5% CO₂) for up to 72 h. Cell viability, proliferation, H₂O₂ production and angiogenesis were analyzed. Sex-specific differences in the expression of VEGFR2 and modulation of NF-kappa B pathway w

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5423444 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature