Model-based analysis of N-glycosylation in Chinese hamster ovary cells.

ABSTRACT: The Chinese hamster ovary (CHO) cell is the gold standard for manufacturing of glycosylated recombinant proteins for production of biotherapeutics. The similarity of its glycosylation patterns to the human versions enable the products of this cell line favorable pharmacokinetic properties and lower likelihood of causing immunogenic responses. Because glycan structures are the product of the concerted action of intracellular enzymes, it is difficult to predict a priori how the effects of genetic manipulations alter glycan structures of cells and therapeutic properties. For that reason, quantitative models able to predict glycosylation have emerged as promising tools to deal with the complexity of glycosylation processing. For example, an earlier version of the same model used in this study

SUBMITTER: Krambeck FJ 

PROVIDER: S-EPMC5423595 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature