Staphylococcus aureus sqr Encodes a Type II Sulfide:Quinone Oxidoreductase and Impacts Reactive Sulfur Speciation in Cells.

ABSTRACT: Recent studies implicate hydrogen sulfide (H₂S) oxidation as an important aspect of bacterial antibiotic resistance and sulfide homeostasis. The cst operon of the major human pathogen Staphylococcus aureus is induced by exogenous H₂S stress and encodes enzymes involved in sulfide oxidation, including a group I flavoprotein disulfide oxidoreductase sulfide:quinone oxidoreductase (SQR). In this work, we show that S. aureus SQR catalyzes the two-electron oxidation of sodium sulfide (Na₂S) into sulfane sulfur (S⁰) when provided flavin adenine dinucleotide and a water-soluble quinone acceptor. Cyanide, sulfite, and coenzyme A (CoA) are all capable of functioning as the S⁰ acceptor in vitro. This activity requires a C167-C344 disulfide bond

SUBMITTER: Shen J 

PROVIDER: S-EPMC5423654 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature