Antibody Distance from the Cell Membrane Regulates Antibody Effector Mechanisms.

ABSTRACT: Immunotherapy using mAbs, such as rituximab, is an established means of treating hematological malignancies. Abs can elicit a number of mechanisms to delete target cells, including complement-dependent cytotoxicity, Ab-dependent cellular cytotoxicity, and Ab-dependent cellular phagocytosis. The inherent properties of the target molecule help to define which of these mechanisms are more important for efficacy. However, it is often unclear why mAb binding to different epitopes within the same target elicits different levels of therapeutic activity. To specifically address whether distance from the target cell membrane influences the aforementioned effector mechanisms, a panel of fusion proteins consisting of a CD20 or CD52 epitope attached to various CD137 scaffold molecules was generated. T

SUBMITTER: Cleary KLS 

PROVIDER: S-EPMC5424080 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature