Direct visualization of Bcl-2 family protein interactions using live cell fluorescent protein redistribution assays.

ABSTRACT: Bcl-2 family proteins have important roles in tumor initiation, progression and resistance to therapy. Pro-survival Bcl-2 proteins are regulated by their interactions with pro-death BH3-only proteins making these protein-protein interactions attractive therapeutic targets. Although these interactions have been extensively characterized biochemically, there is a paucity of tools to assess these interactions in cells. Here, we address this limitation by developing quantitative, high throughput microscopy assays to characterize Bcl-2 and BH3-only protein interactions in live cells. We use fluorescent proteins to label the interacting proteins of interest, enabling visualization and quantification of their mitochondria-localized interactions. Using tool compounds, we demonstrate the suitabilit

SUBMITTER: Wong C 

PROVIDER: S-EPMC5424096 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature