Different Roles of Mitochondrial Calcium Uniporter Complex Subunits in Growth and Infectivity of Trypanosoma cruzi.

ABSTRACT: Trypanosoma cruzi is the agent of Chagas disease, and the finding that this parasite possesses a mitochondrial calcium uniporter (TcMCU) with characteristics similar to that of mammalian mitochondria was fundamental for the discovery of the molecular nature of MCU in eukaryotes. We report here that ablation of TcMCU, or its paralog TcMCUb, by clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 led to a marked decrease in mitochondrial Ca²⁺ uptake without affecting the membrane potential of these cells, whereas overexpression of each gene caused a significant increase in the ability of mitochondria to accumulate Ca²⁺ While TcMCU-knockout (KO) epimastigotes were viable and able to differentiate into trypomastigotes, infect

SUBMITTER: Chiurillo MA 

PROVIDER: S-EPMC5424207 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature