GRHL3 binding and enhancers rearrange as epidermal keratinocytes transition between functional states.

ABSTRACT: Transcription factor binding, chromatin modifications and large scale chromatin re-organization underlie progressive, irreversible cell lineage commitments and differentiation. We know little, however, about chromatin changes as cells enter transient, reversible states such as migration. Here we demonstrate that when human progenitor keratinocytes either differentiate or migrate they form complements of typical enhancers and super-enhancers that are unique for each state. Unique super-enhancers for each cellular state link to gene expression that confers functions associated with the respective cell state. These super-enhancers are also enriched for skin disease sequence variants. GRHL3, a transcription factor that promotes both differentiation and migration, binds preferentially to super-

SUBMITTER: Klein RH 

PROVIDER: S-EPMC5425218 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature