In vivo mapping of tissue- and subcellular-specific proteomes in Caenorhabditis elegans.

ABSTRACT: Multicellular organisms are composed of tissues that have distinct functions requiring specialized proteomes. To define the proteome of a live animal with tissue and subcellular resolution, we adapted a localized proteomics technology for use in the multicellular model organism Caenorhabditis elegans. This approach couples tissue- and location-specific expression of the enzyme ascorbate peroxidase (APX), which enables proximity-based protein labeling in vivo, and quantitative proteomics to identify tissue- and subcellular-restricted proteomes. We identified and localized more than 3000 proteins from strains of C. elegans expressing APX in either the nucleus or cytoplasm of the intestine, epidermis, body wall muscle, or pharyngeal muscle. We also identified several hundred pro

SUBMITTER: Reinke AW 

PROVIDER: S-EPMC5425238 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature