A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae.

ABSTRACT: The budding yeast Saccharomyces cerevisiae divides asymmetrically, with a smaller daughter cell emerging from its larger mother cell. While the daughter lineage is immortal, mother cells age with each cell division and have a finite lifespan. The replicative ageing of the yeast mother cell has been used as a model to study the ageing of mitotically active human cells. Several microfluidic platforms, which use fluid flow to selectively remove daughter cells, have recently been developed that can monitor cell physiology as mother cells age. However, these platforms are not trivial to set up and users often require many hours of training. In this study, we have developed a simple system, which combines a commercially available microfluidic platform (the CellASIC ONIX Microfluidic Platf

SUBMITTER: Cabrera M 

PROVIDER: S-EPMC5425278 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature