Simultaneous Determination of Two Subdomain Folding Rates Using the "Transfer-Quench" Method.

ABSTRACT: The investigation of the mechanism of protein folding is complicated by the context dependence of the rates of intramolecular contact formation. Methods based on site-specific labeling and ultrafast spectroscopic detection of fluorescence signals were developed for monitoring the rates of individual subdomain folding transitions in situ, in the context of the whole molecule. However, each site-specific labeling modification might affect rates of folding of near-neighbor structural elements, and thus limit the ability to resolve fine differences in rates of folding of these elements. Therefore, it is highly desirable to be able to study the rates of folding of two or more neighboring subdomain structures using a single mutant to facilitate resolution of the order and interdependence of such

SUBMITTER: Rahamim G 

PROVIDER: S-EPMC5425376 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature