HN1 contributes to migration, invasion, and tumorigenesis of breast cancer by enhancing MYC activity.

ABSTRACT:

Background

Hematological and neurological expressed 1 (HN1) is upregulated in many tumors, but the role of HN1 in breast cancer progression and its regulatory mechanism have not been well understood.

Methods

To study the role of HN1 in the initiation and progression of breast cancer, we examined HN1 levels in breast cancer cells and tissues and analyzed the relationship between HN1 levels and patient survival. We used mammosphere formation assay, side population analysis, wound healing assay, transwell assay, soft agar formation assay, and xenografted tumor model to determine the effect of HN1 on the expansion of breast cancer stem cells, and the migration, invasion and tumorigenesis of breast cancer. To determine whether HN1 regulates MYC, we used quantitative real-time PCR

SUBMITTER: Zhang C 

PROVIDER: S-EPMC5426009 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature