Robust long-read native DNA sequencing using the ONT CsgG Nanopore system.

ABSTRACT: Background: The ability to obtain long read lengths during DNA sequencing has several potentially important practical applications. Especially long read lengths have been reported using the Nanopore sequencing method, currently commercially available from Oxford Nanopore Technologies (ONT). However, early reports have demonstrated only limited levels of combined throughput and sequence accuracy. Recently, ONT released a new CsgG pore sequencing system as well as a 250b/s translocation chemistry with potential for improvements. Methods: We made use of such components on ONTs miniature 'MinION' device and sequenced native genomic DNA obtained from the near haploid cancer cell line HAP1. Analysis of our data was performed utilising recently described computational tools tailored

SUBMITTER: Carter JM 

PROVIDER: S-EPMC5426553 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature