5α-reductase 1 mRNA levels are positively correlated with TRAMP mouse prostate most severe lesion scores.

ABSTRACT:

Background

The contribution of 5α-reductase 1 and 5α-reductase 2 to prostate cancer development and progression is not clearly understood. TRAMP mice are a common prostate cancer model, in which 5α-reductase 1 and 5α-reductase 2 expression levels, along with prostate lesions scores, have not been investigated at different time points to further understand prostate carcinogenesis.

Method/principal findings

To this end, 8-, 12-, 16-, and 20-week-old male C57BL/6TRAMP x FVB mice prostate most severe and most common lesion scores, 5α-reductase 1 and 5α-reductase 2 in situ hybridization expression, and Ki-67, androgen receptor, and apoptosis immunohistochemistry levels were measured. Levels of these markers were quantified in prostate epithelium, hyperplasia, and tumors sections.

SUBMITTER: Opoku-Acheampong AB 

PROVIDER: S-EPMC5426600 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature