Antibody-nanoparticle conjugates to enhance the sensitivity of ELISA-based detection methods.

ABSTRACT: Accurate antigen detection is imperative for clinicians to diagnose disease, assess treatment success, and predict patient prognosis. The most common technique used for the detection of disease-associated biomarkers is the enzyme linked immunosorbent assay (ELISA). In an ELISA, primary antibodies are incubated with biological samples containing the biomarker of interest. Then, detectible secondary antibodies conjugated with horseradish peroxidase (HRP) bind the primary antibodies. Upon addition of a color-changing substrate, the samples provide a colorimetric signal that directly correlates to the targeted biomarker concentration. While ELISAs are effective for analyzing samples with high biomarker content, they lack the sensitivity required to analyze samples with low antigen levels. We h

SUBMITTER: Billingsley MM 

PROVIDER: S-EPMC5426781 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature