The host ubiquitin-dependent segregase VCP/p97 is required for the onset of human cytomegalovirus replication.

ABSTRACT: The human cytomegalovirus major immediate early proteins IE1 and IE2 are critical drivers of virus replication and are considered pivotal in determining the balance between productive and latent infection. IE1 and IE2 are derived from the same primary transcript by alternative splicing and regulation of their expression likely involves a complex interplay between cellular and viral factors. Here we show that knockdown of the host ubiquitin-dependent segregase VCP/p97, results in loss of IE2 expression, subsequent suppression of early and late gene expression and, ultimately, failure in virus replication. RNAseq analysis showed increased levels of IE1 splicing, with a corresponding decrease in IE2 splicing following VCP knockdown. Global analysis of viral transcription showed the expression

SUBMITTER: Lin YT 

PROVIDER: S-EPMC5426786 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature