Pregnancy Reprograms Large-Conductance Ca²⁺-Activated K⁺ Channel in Uterine Arteries: Roles of Ten-Eleven Translocation Methylcytosine Dioxygenase 1-Mediated Active Demethylation.

ABSTRACT: The large-conductance Ca²⁺-activated K⁺ (BK_Ca) channel is of critical importance in pregnancy-mediated increase in uterine artery vasodilation and blood flow. The present study tested the hypothesis that active DNA demethylation plays a key role in pregnancy-induced reprogramming and upregulation of BK_Ca channel β1 subunit (BKβ1) in uterine arteries. Uterine arteries were isolated from nonpregnant and near-term pregnant sheep. Pregnancy significantly increased the expression of ten-eleven translocation methylcytosine dioxygenase 1 (TET1) in uterine arteries. A half-palindromic estrogen response element was identified at the TET1 promoter, and estrogen treatment increased TET1 promoter activity and TET1 expression in uterine arteries. In accordanc

SUBMITTER: Hu XQ 

PROVIDER: S-EPMC5426984 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature