Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxin_H35L in Escherichia coli.

ABSTRACT: Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides play an important role in targeting proteins for translocation across the cytoplasmic membrane to the periplasmic space and release into culture medium during the secretion process. Alpha toxin_H35L (AT_H35L) was selected as an antigen for vaccine development against Staphylococcus aureus infections. It was successfully secreted into culture medium of E. coli by using bacterial signal peptides linked to the N-terminus of the protein. In order to improve the level of secreted AT_H35L, we designed a series of novel sign

SUBMITTER: Han S 

PROVIDER: S-EPMC5427057 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature