Quantitative analysis of the cysteine redoxome by iodoacetyl tandem mass tags.

ABSTRACT: The redox conditions that reign inside a cell have a determining effect on a number of biological processes. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are key redox players and have been linked to a number of pathologies. They have also been shown to play an important regulating role in cell signaling events. On the proteome level, thiol groups of cysteinyl side chains constitute the major targets of ROS and RNS. A number of analytical techniques based on mass spectrometry have been developed to characterize the cysteine redoxome, often facing a number of technical challenges, mostly related to the lability, heterogeneity, and low abundance of the oxidized forms. Furthermore, any posttranslational modification (PTM) quantification method needs to take the parent pro

SUBMITTER: Shakir S 

PROVIDER: S-EPMC5427158 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature