Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.

ABSTRACT: Human pluripotent stem cells (hPS cells) can self-renew indefinitely, making them an attractive source for regenerative therapies. This expansion potential has been linked with the acquisition of large copy number variants that provide mutated cells with a growth advantage in culture. The nature, extent and functional effects of other acquired genome sequence mutations in cultured hPS cells are not known. Here we sequence the protein-coding genes (exomes) of 140 independent human embryonic stem cell (hES cell) lines, including 26 lines prepared for potential clinical use. We then apply computational strategies for identifying mutations present in a subset of cells in each hES cell line. Although such mosaic mutations were generally rare, we identified five unrelated hES cell lines that car

SUBMITTER: Merkle FT 

PROVIDER: S-EPMC5427175 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature