NADPH oxidase 1 supports proliferation of colon cancer cells by modulating reactive oxygen species-dependent signal transduction.

ABSTRACT: Reactive oxygen species (ROS) play a critical role in cell signaling and proliferation. NADPH oxidase 1 (NOX1), a membrane-bound flavin dehydrogenase that generates O₂^̇̄, is highly expressed in colon cancer. To investigate the role that NOX1 plays in colon cancer growth, we used shRNA to decrease NOX1 expression stably in HT-29 human colon cancer cells. The 80-90% decrease in NOX1 expression achieved by RNAi produced a significant decline in ROS production and a G₁/S block that translated into a 2-3-fold increase in tumor cell doubling time without increased apoptosis. The block at the G₁/S checkpoint was associated with a significant decrease in cyclin D₁ expression and profound inhibition of mitogen-activated protein kinase (MAPK) si

SUBMITTER: Juhasz A 

PROVIDER: S-EPMC5427267 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature