Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129.

ABSTRACT:

Background

Herpes simplex virus type 1 strain 129 (H129) has represented a promising anterograde neuronal circuit tracing tool, which complements the existing retrograde tracers. However, the current H129 derived tracers are multisynaptic, neither bright enough to label the details of neurons nor capable of determining direct projection targets as monosynaptic tracer.

Methods

Based on the bacterial artificial chromosome of H129, we have generated a serial of recombinant viruses for neuronal circuit tracing. Among them, H129-G4 was obtained by inserting binary tandemly connected GFP cassettes into the H129 genome, and H129-ΔTK-tdT was obtained by deleting the thymidine kinase (TK) gene and adding tdTomato coding gene to the H129 genome. Then the obtained viral tracers were te

SUBMITTER: Zeng WB 

PROVIDER: S-EPMC5427628 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature