Watasemycin biosynthesis in <i>Streptomyces venezuelae</i>: thiazoline C-methylation by a type B radical-SAM methylase homologue.

ABSTRACT: 2-Hydroxyphenylthiazolines are a family of iron-chelating nonribosomal peptide natural products that function as virulence-conferring siderophores in various Gram-negative bacteria. They have also been reported as metabolites of Gram-positive Streptomyces species. Transcriptional analyses of Streptomyces venezuelae ATCC 10712 revealed that its genome contains a putative 2-hydroxyphenylthiazoline biosynthetic gene cluster. Heterologous expression of the gene cluster in Streptomyces coelicolor M1152 showed that the mono- and dimethylated derivatives, thiazostatin and watasemycin, respectively, of the 2-hydroxyphenylthiazoline enantiopyochelin are two of its metabolic products. In addition, isopyochelin, a novel isomer of pyochelin containing a C-methylated thiazolidine,

SUBMITTER: Inahashi Y 

PROVIDER: S-EPMC5427693 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature