RNA-Sequencing data supports the existence of novel VEGFA splicing events but not of VEGFA_xxxb isoforms.

ABSTRACT: Vascular endothelial growth factor (VEGFA), a pivotal regulator of angiogenesis and valuable therapeutic target, is characterised by alternative splicing which generates three principal isoforms, VEGFA₁₂₁, VEGFA₁₆₅ and VEGFA₁₈₉. A second set of anti-angiogenic isoforms termed VEGFA_xxxb that utilise an alternative splice site in the final exon have been widely reported, with mRNA detection based principally upon RT-PCR assays. We sought confirmation of the existence of the VEGFA_xxxb isoforms within the abundant RNA sequencing data available publicly. Whilst sequences derived specifically from each of the canonical VEGFA isoforms were present in many tissues, there were no sequences derived from VEGFA_xxxb isoforms. Sequencing

SUBMITTER: Bridgett S 

PROVIDER: S-EPMC5427905 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature