Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O₂ reducing enzyme and an adhesin.

ABSTRACT: Mycoplasma bovis causes considerable economic losses in the cattle industry worldwide. In mycoplasmal infections, adhesion to the host cell is of the utmost importance. In this study, the amino acid sequence of NOX was predicted to have enzymatic domains. The nox gene was then cloned and expressed in Escherichia coli. The enzymatic activity of recombinant NOX (rNOX) was confirmed based on its capacity to oxidize NADH to NAD⁺ and reduce O₂ to H₂O₂. The adherence of rNOX to embryonic bovine lung (EBL) cells was confirmed with confocal laser scanning microscopy, enzyme-linked immunosorbent assay, and flow cytometry. Both preblocking EBL cells with purified rNOX and preneutralizing M. bovis with polyclonal antiserum to rNOX significantly reduced the

SUBMITTER: Zhao G 

PROVIDER: S-EPMC5427908 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature